Bugcount® Fuel XL

For further information, please visit my.luminultra.com

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Getting started

Measure Background and Luminase enzyme strength

This step only needs to be done once per testing session, or upon opening a new bottle of enzyme.

Personal protection equipment (e.g., nitrile gloves, safety glasses, etc.) is recommended to be worn for the entire procedure.

• Turn on the luminometer.
• Empty the Luminase Buffer into the vial of Luminase XL Enzyme vial and wait 5 minutes for the solution to dissolve.
• Using a fresh exact volume pipette, add the rehydrated Luminase XL Enzyme solution to a new, clean test tube. Place this test tube into the luminometer and take a reading.
• Record the result in the table provided as B Background RLU. This reading should always be less than 100 RLU.
• Remove test tube from the luminometer, add 2 drops of UltraCheck 1, then swirl to mix it with the Luminase XL Enzyme solution.
• Place this test tube into the luminometer, close the lid, and take a reading.
• Record the result in the table provided as C ATP1 RLU. This reading should always be greater than 50,000. If the reading is below 50,000, contact Customer Service.

If the background is above 100,000 RLU, visit FAQs at my.luminultra.com for troubleshooting steps. If backgrounds above 100,000 RLU persist, contact help@luminultra.com.

Using the exact volume pipettes

• Ensure pipette tips do not come in contact with any surface prior to use.
• Squeeze the top bulb, dip the stem into the liquid and release the bulb.
• Squeeze the top bulb again to release the liquid.
• The pipette is designed to transfer the correct amount of liquid. Any excess will remain in the pipette.
• Discard after use.

Cellular ATP Analysis

2.1. Clean – Remove interferences

• Re-attach the filter to the syringe.
• Empty a LumiClean tube into the syringe.
• Use the plunger to slowly push the LumiClean through the filter into a waste container.
• Detach the filter, then remove the plunger from the syringe.

Tip: Relay™ and the Bugcount analyzer can do the work For you

When all inputs are entered in Relay’s Run tests workflow, the results will calculate automatically. Select Save Test to catalog and share your results with your team. Or, run the test on the Bugcount analyzer and import the results to Relay for sharing.

Alternatively, you can manually calculate your test result using one of the simple calculations shown below corresponding to your sample type. This calculation will convert the RLU output of the luminometer to cellular ATP (cATP), which is reported in pg/mL as your final test results. If performing more than 3 tests, copy the table below into a spread sheet or notebook in order to keep results and calculations organized.

Calculate your result based on the following equation:

\textit{Result (cATP)} =

{

\frac{

\colorbox{#72BF44}{\color{white}A}

-

\colorbox{#F15A24}{\color{white}B}

}{

\colorbox{#8E5BAA}{\color{white}C}

}}

\times 200
 

For sample volumes outside of those recommended in Step 1, use the following equation where V represents the actual sample volume filtered:

\mathrm{Result (cATP)}\
=
{

\frac{

\colorbox{#72BF44}{\color{white}A}

-

\colorbox{#F15A24}{\color{white}B}

}{

\colorbox{#8E5BAA}{\color{white}C}

}}
\times
\frac{10{,}000\ }{V_{\mathrm{sample}}\ \mathrm{(mL)}}

The categorical rating thresholds (for example 1.5 pg/mL) provided in this are based on general experience with microbiological contamination in fuel systems. It is recommended that test kit users develop a record of the relationship between ATP test results and other observations to develop categorical thresholds that meet their specific needs.

Support